Product Description: Price

Gamma-aminobutyrate aminotransferase (GABAT or ABAT) is a pyridoxal phosphate-dependent enzyme involved in the degradation of the inhibitory neurotransmitter GABA. GABAT converts GABA and alpha-ketoglutarate to glutamate and succinic semialdehyde. The fine balance of the GABAergic inhibitory tone in the CNS is critical for countering the excitatory pathway mediated by L-glutamate. Thus, therapeutic targeting of the GABA system covers a broad spectrum of neurological and psychiatric disorders, and GABAT is a validated target for epilepsy therapy because inhibition of the enzyme raises GABA concentrations in the brain. GABAT has been found in a wide variety of species (microorganisms, plants and invertebrates) in addition to vertebrates. GABAT activity is also demonstrable in hepatic tissue in addition to brain. GABAT deficiency is listed as a “rare disease" by the Office of Rare Disease of the NIH. The GABAT Assay Kit is based on sequential transamination reaction and glutamate dehydrogenase reaction, which couples the reduction of INT to INT-formazan (molar extinction coefficient = 18/mM-cm at 492 nm), allowing for sensitive detection of GABAT enzyme activity in crude tissue samples. Assay solutions are stable for several years if stored and handled properly.

#GABA aminotransferase #GABAT #GABA #transamination #CNS

Kit Components:

GABAT Substrate: 5 ml (100 assays), store at -80°C

TA Assay Solution: 5 ml, store at -80°C

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Tris

Related Kits:

AST Assay, ALT Assay

Citation:

Besse et al

Personalized medicine approach confirms a milder case of ABAT deficiency

Molecular Brain 9: 93, 2016

Zhao et al

High methylation of the 4-aminobutyrate aminotransferase gene predicts a poor prognosis in patients with myelodysplastic syndrome

International J of Oncology 54: 491-504, 2019