Product Description: Price

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme mediating the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate with the production of NADH. 1,3,-Bisphosphate glycerate, being a high-energy intermediate, is then used for ATP production via substrate-level phosphorylation. Although this house-keeping gene is often used as internal control for RNA and protein analysis, expression of the gene can be altered under certain cell growth and differentiation conditions. In addition, a switch from aerobic metabolism to anaerobic glycolysis may also be accompanied by a change in GAPDH expression. The GAPDH enzyme activity assay kit can be used to quickly assess potential changes in the expression of the gene. The non-radioactive colorimetric GAPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GAPDH activity. The kit is stable for at least one year if handled and stored properly.

#Glyceraldehyde-3-phosphateDehydrogenase #GAPDH #glycolysis #NADH #GAP​

Kit Components:

GAPDH Assay Solution: 5 ml, store at -80°C

50x GAPDH Substrate- 0.1 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Tris

Related Kits:

Hexokinase Assay, Glucose Assay, L-Lactate Assay, D-Lactate Assay

Citation:

Giulivi et al

Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice

J Biol Chem, 286:99-113, 2011

Missihoun et al

Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of delta-sarcoglycan-null cardiomyopathy

Molecular and Cellular Biochemistry 321:45-52, 2009