Product Description:

Glutamate dehydrogenase (GLDH), an important mitochondrial matrix enzyme integrating carbon and nitrogen metabolism, catalyzes the reversible oxidative deamination of L-glutamate into free ammonia and alpha-ketoglutarate in all tissues. Since the forward reaction generates a TCA cycle intermediate, GLDH is involved in energy homeostasis. In addition, kidney GLDH participates in acid/base balance of blood by release of ammonia. In clinical diagnostics, increased circulating levels of GLDH signal toxic or hypoxic liver damage. Our non-radioactive colorimetric GLDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GLDH activity. The assay is simple and non-radioactive. Each kit is sufficient for 100 microassays. The assay solution should be kept in aliquots at -80ºC, and will remain stable for many years at -80ºC.

#Glutamate Dehydrogenase​

Kit Components:

GLDH Assay Solution: 5 ml, store at -80ºC (100 wells)

10x GLDH Substrate: 0.5 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Hepes

Related Kits:

L-Glutamate Assay

Citation:

Park et al

Identification of multiple Cryptococcal fungicidal drug targets by combined gene dosing and drug affinity responsive target stability screening

mBIO 7:e01073, 2016