BMR Glutaminase Assay Kit

Product Description: Price

Glutaminase (glutamine amidohydrolase or GLS) catalyzes the following reaction: Glutamine + H2O → Glutamate + NH3. The enzyme has tissue-specific roles in multiple organs. Two different mammalian phosphate-activated GLS isoforms are known: GLS1 (kidney- type) and GLS2 (liver-type a target of the tumor suppressor protein p53). The hydrolytic activity of glutaminase generates ammonia for urea synthesis in the liver similar to that mediated by glutamate dehydrogenase. During renal acidosis, glutaminase is induced in the kidney, leading to increased excretion of ammonia, which plays an important role in maintaining acid-base homeostasis. Glutaminase regulates the levels of the neurotransmitter glutamate in the brain. The rate of glutaminolysis is known to increase in tumors and may be a hot spot for regulation of cancer cell metabolism. Inhibitors of GLS may therefore be candidate drugs for cancer therapy. The GLS Assay Kit is based on sequential GLS-mediated hydrolytic reaction and glutamate dehydrogenase reaction, which is coupled to reduction of INT to INT-formazan. The increase in optical density at 492 nm is proportional to GLS enzyme activity. The assay solution is stable for several years if stored and handled properly.
#Glutaminase #GLS #glutamine #ammonia

Kit Components:

GLS Buffer: 10 ml, store at -80°C
Glutamine: 0.3 g, store at 4ºC

TA Assay Solution: 5 ml (100 wells), store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

Biomedical Research Service

Glutaminase Assay Kit

(GLS Assay Kit)