Product Description: Price

The glutathione S-transferase (GST) family comprises multiple classes of abundant enzymes with highly specific and overlapping functions. More than 20 GST class members have been identified in Homo sapiens. GSTs are well known for their ability to catalyze the conjugation of reduced glutathione (GSH) to electrophiles on many substrates for the purpose of detoxification. GSTs also catalyze GSH-dependent isomerization reactions involved in the synthesis of several prostaglandins and steroid hormones. In addition, GSTs have been implicated in cell signaling, oxidative stress, cancer development, neuronal degeneration and chemotherapeutic drug resistance. Most GSTs interact with the compound 1-chloro-2,4-dinitrobenzene (CDNB), forming the basis for a spectrophotometric assay for GST activity (Habig et al., PNAS 71:3879-3882, 1974). The GST Assay Kit is based on the use of CDNB, and can be employed to measure the activity of a purified GST enzyme or total GST activity of a crude cell/tissue extract. GSH conjugation to CDNB causes an increase in the absorbance at 340 nm. The assay requires the use of UV-transparent 96-well plates for measuring kinetic changes in O.D.340 nm. Each assay kit is for 250 assays.

#Glutathione-S-transferase​ #GST #GSH #CDNB #detoxification

Kit Components:

10x Cell Lysis Solution: 25 ml, store at 4ºC

50x GSH Buffer: 1 ml, store at 4°C

200x CDNB: 0.25 ml, store at -20°C (for 250 assays)

GSH: 0.35 g, store at 4ºC

SDS:

Tris, CDNB, DMSO

Related Kits:

Glutathione Assay, Free Thiol Assay