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Isocitrate Dehydrogenase 1/2 Assay Kit



(IDH 1/2 Assay Kit)



Product Description:

Isocitrate dehydrogenase (IDH) is an essential enzyme of the Krebs cycle. There are three isoforms: IDH1 (peroxisomes and cytosol NADP dependent), IDH2 (mitochondria NADP dependent), and IDH3 (mitochondria NAD dependent). The enzymes catalyze oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG) with generation of carbon dioxide and NADH or NADPH. IDH catalyzes one of the irreversible reactions in the cycle and therefore must be regulated to avoid unnecessary depletion of isocitrate, i.e., the enzyme is allosterically activated by ADP and inhibited by ATP or NADH/NADPH. IDH mutations are prevalent especially in chondrosarcoma, glioma, acute myeloid leukemia, and angioimmunoblastic lymphomas, and these mutations are considered gain-of-function, leading to increased conversion of α-KG to 2-hydroxyglutarate, which can affect DNA methylation, histone methylation, receptor signaling, and cell growth. Since limited treatment options are available for these tumors, pharmacological IDH targeting has been investigated as potential effective treatments. The IDH enzyme activity assay is based on the reduction of INT in a NAD+- or NADP+-coupled reaction to formazan, which exhibits an absorption maximum at 492 nm (Molar extinction coefficient=18 mM-1cm-1) and allows for sensitive measurement of IDH activity. Reagents are stable for several years if stored and handled properly.

​#Isocitrate Dehydrogenase

Kit Components:

IDH 1/2 Assay Solution: 5 ml, store at -80°C (for 100 wells)

10x IDH Substrate: 0.5 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Hepes


Related Kits:

MDH Assay Kit