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Inosine-5'-monophosohate Dehydrogenase Assay Kit



(IMPDH Assay Kit)



Product Description:

Inosine-5′-monophosphate dehydrogenase (IMPDH) is a purine metabolic enzyme catalyzing the first and rate-limiting step towards the synthesis of guanosine triphosphate (GTP). IMPDH converts inosine monophosphate (IMP) to xanthosine monophosphate (XMP) using NAD+ as a cofactor, providing precursors for DNA and RNA bioysynthesis. The protein has an additional role as a DNA-binding transcriptional repressor. Since IMPDHs are known to exhibit species-specific kinetic properties and inhibitor sensitivities, the enzymes have been the target of antiviral, antibacterial and anticancer therapeutics. The measurement of IMPDH activity thus serves as a useful tool for drug design and screening. Indeed, several pharmaceutical companies have used the kit for their drug screening application. The IMPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to INT-formazan. The intensity of the red color formed is increased in the presence of increased activity of IMPDH1 or IMPDH2. The assay solution should be stored in aliquots at -80ºC.

#Inosine-5'-monophosohate Dehydrogenase


Kit Components:

IMPDH Assay Solution: 5 ml, store at -80°C (for 100 wells)

50x IMPDH Substrate: 0.1 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC


SDS:

TX-100, DMSO, INT, Hepes


Related Kits:

HK Enzyme Assay, G6PD Enzyme Assay, Adenylate Kinase Assay


Citation:

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity

Cell Division 13:5, 2018