Product Description: Price

Monoamine oxidases (MAOs) are mitochondrial flavoenzymes that oxidatively deaminate neurotransmitters such as dopamine, serotonin, and epinephrine and drugs such as phenylephrine and phenelzine. Catalytically, the amine is first oxidized to the corresponding imine with reduction of FAD, and O2 accepts electrons from FADH2, forming hydrogen peroxide. The imine is then hydrolyzed to form ammonia and aldehyde. The reaction products are therefore the corresponding aldehyde, ammonia, and hydrogen peroxide. MAO-A is involved in the metabolism of dopamine, serotonin, noradrenaline whereas MAO-B mainly metabolizes dopamine and amine xenobiotics. The differences between the substrate selectivity of MAO-A and MAO-B are clinically significant in that MAO-A inhibitors are typically used in the treatment of depression and MAO-B inhibitors are useful for the treatment of Parkinson's disease (PD). If MAO-B is inhibited, more dopamine is available for PD patients and hence the therapeutic benefits. The assay kit uses dopamine as substrate and therefore detects the activities of both MAO enzymes. Potassium iodide (KI) is used for colorimetric detection of hydrogen peroxide at 400-405 nm (molar extinction coefficient 5.3/mM-cm). Each kit is sufficient for ~50 samples using a 96-well format.

#monoamine #monoamine oxidase #MAOI #neurotransmitters #dopamine #epinephrine #norepinephrine #tyramine

Kit Components:

MAO Substrate: 15 mg (50 samples), store at 4°C

MAO Control: 4 ml, store at -20°C

Detection reagent: 15 g, store at 4°C

10x Tissue Lysis Solution: 25 ml, store at 4°C

SDS:

KI, NaN3, Tris, TX-100, EDTA

Related Kits:

GABAT Assay, Xanthine Dehydrogenase, Aldehyde Oxidase, Xanthine Oxidase