Product Description:

Cells exposed to oxidative stress accumulate a complex profile of aldehydes which are derived from the decomposition of lipid hydroperoxides (Free Radical Biology & Medicine 7:197,1989). In contrast to free radicals, also caused by oxidative stress, the aldehydes are more stable and tend to diffuse over large distances both intracellularly and extracellularly. Thus, profound cytotoxic and genotoxic effects are associated with these aldehyde products, and the determination of aldehydes is of wide interests. Malondialdehyde (MDA) and MDA-like substances are by far the most abundant lipid peroxidation products. High levels of MDA and MDA-like substances are produced during the oxidation of low density lipoprotein. Although HPLC can be used to measure the production of these lipid peroxidation products, the analysis of MDA by the classical TBA (2-thiobarbituric acid) test remains a simple, reliable, and useful method applicable in most if not every laboratory (J. Lipid Res. 28:495,1987). Our Lipid Peroxidation Assay Kit is formulated based on the TBA method. The kit is stable for one year if stored and handled properly.

Kit Components:

TBA: 0.75 g, store at room temperature

MDA Assay Solution: 100 ml, store at room temperature

MSDS:

NaOH, 2-thiobarbituric acid, TCA

Related Kits:

Cell Injury Assay, Peroxide Assay, Nitric Oxide Assay,

Cell Viability Assay, Free Thiol Assay

Citation:

Yoo et al

Delineating the Role of Glutathione Peroxidase 4 in Protecting Cells Against Lipid Hydroperoxide Damage and in Alzheimer’s Disease

ANTIOXIDANTS & REDOX SIGNALING 12:819-827, 2010

Missihoun et al

Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of delta-sarcoglycan-null cardiomyopathy

Mol Cell Biochemistry 321:45, 2009