Product Description: Price

The human lysosomal enzyme α-NAGAL (or Nagalase E.C. 3.2.1.49) removes O-linked α-GalNAc from glycolipids and glycoproteins. In lysosomal storage disease (LSD), enzyme deficiency in a patient leads to the accumulation of substrate in the tissues, resulting in the development of clinical symptoms. Schindler/Kanzaki disease, a rare LSD caused by deficient Nagalase, displays several phenotypes from severe infantile neurodegeneration to mild cognitive impairments. Individuals with defective Nagalase can abnormally process their blood group antigens. Notably, recent cancer research has suggested that Nagalase facilitates the deglycosylation of Macrophages Activator Factor (Gc- MAF), which in turn inhibits the activation of macrophages. Since macrophages play a vital role in the fight against cancer cells, measurement of Nagalase activity bears clinical significance. The Nagalase Assay Kit is based on the enzymatic cleavage of the artificial substrate p-nitrophenyl- alpha-N-acetyl-galactosaminide to nitrophenol in an acidic buffer (Marine Drugs 16, 155, 2018). Ionization of nitrophenol by NaOH produces a yellow color exhibiting an absorption maximum at 405-415 nm, which allows for sensitive detection of Nagalase activity in crude cell/tissue extracts, serum/plasma and urine. The assay is designed for the 96-well plate format. Repeated freeze-thaw cycles of the assay solution should be avoided.

​#Nagalase #lysosome #Schindler/KanzakiDisease #α-NAGAL

Kit Components:

10x Cell Lysis Solution: 25 ml, store at 4ºC
Nagalase Assay Solution: 5 ml (100 wells), store at -20ºC
Nagalase Control Solution: 5 ml, store at 4ºC

Reaction Stop: 4 ml, store at 4ºC

SDS:

p-nitrophenyl-a-N-acetyl-galactosaminide, TX-100, sodium acetate, acetic acid, NaOH

Related Kits:

OGA Assay