Product Description:
The osteogenesis assay kit is based on the alkaline phosphatase (ALP)-mediated conversion of p-nitrophenol phosphate to nitrophenol in an alkaline buffer. Nitrophenol exhibits an absorption maximum at 405-415 nm. The assay can also be used to examine cell differentiation such as osteogenesis, which is associated with increased expression of ALP. Repeated freeze-thaw cycles of the assay solution should be avoided. The enzyme assay system can be completed in as short as 10 minutes. The method is adaptable to automation.
#Osteogenesis
Kit Components:
10x Cell Lysis Solution: 25 ml, store at RT
ALP Assay Solution: 20 ml (200 wells), store in aliquots at -20ºC
MSDS:
p-nitrophenol phosphate, Tris
Related Kits:
Chondrogenesis Assay, Adipogenesis Assay,
Cell Proliferation Assay
Citation:
Leiker et al
Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells
Cell Transplantation 17:911-922, 2008
