BMR Pyruvate Dehydrogenase Assay Kit

Product Description: Price

Pyruvate is the end product of aerobic glycolysis and is at an important metabolic crossroad. The pyruvate dehydrogenase multi-enzyme complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated by the E1 subunit (pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit (dihydrolipoyl dehydrogenase). The reactions of PDC serve to interconnect the metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs Cycle. As a consequence, PDC is highly regulated by a variety of allosteric effectors and by covalent modification. The importance of PDC in metabolic regulation is evident from the fact that although diseases associated with deficiencies of PDC have been observed, affected individuals often do not survive to maturity. This non-radioactive colorimetric PDC assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased PDC activity. The kit is stable for many years under proper storage and handling conditions.

#PyruvateDehydrogenase #PDC #pyruvate #acetyl-CoA

Kit Components:

PDC Assay Solution: 5 ml (100 wells), store at -80ºC

20x Cofactors 0.25 ml, store at -80ºC

20x PDC Substrate: 0.25 ml, store at -80ºC

10x Lysis Buffer: 25 ml, store at 4ºC

10x Extraction Buffer: 20 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Tris

Citation:

Eastlack et al

Suppression of PDHX by microRNA-27b deregulates cell metabolism and promotes growth in breast cancer

Molecular Cancer 17:100, 2018

Martin et al

Androgen deprivation leads to increased carbohydrate metabolism and hexokinase 2-mediated survival in Pten/Tp53-deficient prostate cancer

Oncogene 36(4):525-533, 2017

Biomedical Research Service

Pyruvate Dehydrogenase Complex Assay Kit

(PDC Assay Kit)