Product Description:

Phosphoglucose isomerase (PGI), or glucose-6-phosphate isomerase (GPI), mediates the interconversion between glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P) in glycolysis and gluconeogenesis. Since the enzymatic step is reversible, the direction of the reaction is determined in part by G6P and F6P concentrations. In addition to its role in glucose metabolism, PGI also functions extracellularly. PGI secreted by cancer cells is known as autocrine motility factor (AMF), which through AMF receptor promotes cancer metastasis. PGI has also been described as a neurotrophic factor and maturation factor in its diverse extracellular function. Clinically, PGI deficiency can lead to hemolytic anemia due to impaired glycolysis in red blood cells, and elevated serum levels of PGI can be a prognostic biomarker for several types of cancers. The PGI activity assay kit is based on the measurement of G6P formed from F6P, and the measurement is coupled to the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. Since the INT reaction product exhibits an absorption maximum at 492 nm, the intensity of the red color formed is increased in response to increased PGI activity. The PGI Assay Solution should be stored at -80ºC, and will remain stable for many years at -80ºC.

Kit Components:

PGI Assay Solution: 5 ml, store at -70°C

20x PGI Substrate: 0.25 ml, store at -70ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS:

TX-100, INT, Hepes, DMSO

Related Kits:

HK/GK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay Pyruvate Assay, L-Lactate Assay

Citation:

Zhu et al

Upregulation of non-canonical Wnt ligands and oxidative glucose metabolism in NASH induced by methionine-choline deficient diet.

Trends in Cell and Molecular Biology 13:47-56, 2018

Lin et al

Secreted phosphoglucose isomerase is a novel biomarker of nonalcoholic fatty liver in mice and humans

BBRC 529:1101, 2020