Product Description:
Quantification of inorganic as well as organic phosphates (such as phospholipids, phosphoproteins, and nucleid acids) is of interest in many biological studies. Phosphatase activity, for instance, can be derived from such an analysis after co-incubation of a phosphatase and a specific phosphopeptide substrate (J. Cell. Biochem. 77:445,2000). Similarly, an assay for phospholipase activity can be performed after hydrolysis of phospholipids by phospholipases. Although various methods can be employed to measure phosphates, non-radioactive colorimetric protocols suitable for high-throughput analysis are the methods of choice in most laboratories. Perhaps the most sensitive colorimetric method for phosphate assay is based on complex formation of phosphomolybdate with the basic dye Malachite Green (Anal. Biochem. 161:45,1987 & 271:29,1999). Our Phosphate Assay Kit (sufficient for 400 assays) is formulated to maximize the sensitivity and stability of the dye-phosphomolybdate reaction. The kit is stable for at least one year if handled and stored properly. The assay, compatible with microplate formats, is simple and can be finished in 15 - 20 min. Reaction product is quantified by absorbance at 600-660 nm.
#Phosphate
Kit Components:
Phosphate Assay Solution: 40 ml, store at 4ºC (for 400 assays)
NaH2PO4 Standard (10 mM): 1 ml, store at 4ºC
MSDS:
Malachite Green, ammonium molybdate
Related Kits:
ATP assay
Citation:
Martin et al
Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts.
Am J Physiol. 309:C139, 2015
Lin et al.
sFRP2 activates Wnt/beta-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling.
Am J. Physiol. 311:C710-C719, 2016
