BMR Pyruvate Kinase Assay Kit

Pyruvate kinase (PK) catalyzes the last step of glycolysis, converting phosphoenolpyruvate (PEP) to pyruvate with production of one ATP. Liver PK is subject to hormonal and allosteric regulation, which is involved in maintaining blood glucose homeostasis. The importance of this substrate-level phosphorylation step is underscored by the finding that PK deficiency, one of the most common enzymopathies, manifests clinically as hemolytic anemia. The clinical severity of PK deficiency varies widely, ranging from a mildly compensated anemia to severe childhood anemia. In addition, PK isoform switch is known to promote tumorigenesis. The enzyme activity assay kit can measure PK activity present in serum/plasma and crude cell/tissue extracts. Luciferase-based detection of ATP produced by PK reaction allows fast, sensitive and quantitative assay of PK activity. Measurement of ATP requires a luminometer. Kit components are stable for many years if stored and handled properly.

#PyruvateKinase #glycolysis #phosphoenolpyruvate #hemolyticanemia

Kit Components:

PK Substrate- 0.3 ml, store at -80ºC

10x Sample Buffer- 25 ml, store at 4ºC

ATP Assay Solution- 10 ml (100 assays), store at -80ºC

TCA Stop- 0.6 ml, store at 4ºC

SDS:

luciferin, Tris, dithiothreitol , Trichloroacetic acid, EDTA

Citation:

Zhu et al

Upregulation of non-canonical Wnt ligands and oxidative glucose metabolism in NASH induced by methionine-choline deficient diet

Trends in Cell and Molecular Biology 13:47-56, 2018

Biomedical Research Service

Pyruvate Kinase Assay Kit

(PK Assay Kit)