Product Description: Price

The detergent compatible Protein Assay Kit combines two famous reactions- the Biuret reaction and the Smith reaction. The Biuret reaction depicts the reduction of cupric ion (Cu2+) to cuprous ion (Cu1+) by protein in an alkaline solution. The intensity of the reaction is proportional to the number of peptide bonds (at least 2 peptide bonds) and therefore to the number of protein molecules present in the sample solution. The Smith reaction depicts the chelation of each Cu1+ ion by two molecules of bicinchoninic acid (BCA), resulting in an intense purple-colored product that absorbs light at 562 nm (Anal. Biochem. 150: 76–85, 1985), which can be measured with a standard laboratory spectrophotometer or microplate reader. Unlike the dye-based protein assay system, the BCA-based protein assay system is compatible with detergent-containing protein samples. However, the BCA assay is incompatible with most divalent ions. Prepare fresh working solution by mixing 50 parts of BCA Solution with 1 part of Copper Solution (50:1). The Protein Assay System has a broad working range of 20 - 2000 ug/ml proteins. The assay kit also includes 1 ml of 100 mg/ml sterilized BSA dissolved in distilled water to be diluted for use as protein standard. The assay kit is stable for at least one year under proper storage and handling conditions.

#bicinchoninicacid #proteinconcentration #Biuretreaction #Smithreaction #BCAassay

Kit Components:

PA Solution A: 2 x 100 ml (2,000 assays), store at RT

PA Solution B: 4 ml, store at RT

100 mg/ml Bovine Serum Albumin (BSA): 1 ml, store at -20ºC

MSDS:

NaOH, BCA, Potassium sodium tartrate, Cupric sulfate

Related Kits:

Protein Assay Kit (EDTA/Mg+2 compatible)

Citation:

Martin et al

Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts

Am J Physiol Cell Physiol, 309:C139, 2015