Product Description:

Pyruvate is metabolized aerobically to acetyl-CoA and anaerobically to lactate (homolactic fermentation) or ethanol (alcoholic fermentation in yeast). Pyruvate is also converted to oxaloacetate by an anaplerotic reaction mediated by pyruvate carboxylase, and the oxaloacetate can be used for replenishing TCA cycle intermediates or promoting gluconeogenesis. Conversion of pyruvate to lactate is obligatory for bacteria, serving to regenerate NAD+, and is enhanced in eukaryotic cells when mitochondrial function is impaired. LDH-A-mediated conversion of pyruvate to lactate by cancer cells in the presence of adequate oxygen supply (aerobic glycolysis) constitutes the central concept of the Warburg hypothesis, and is believed to be the underlying cause of tumorigenesis. The Pyruvate Assay Kit is based on the difference in the UV absorption spectra between the oxidized and reduced forms of NAD+/NADH at 340 nm (detection sensitivity ~20 uM). The assay requires a 0.1 ml Quartz cuvette for measuring kinetic changes in O.D.340 nm. Alternatively, a UV-transparent 96-well plate can be used. The assay solution should be stored in aliquots at -80ºC, and will remain stable for several years at -80ºC.

​#Pyruvate

Kit Components:

Pyruvate Assay Solution: 10 ml (for 100 wells), store at -70°C
NADH: 20 mg, store at -70°C (avoid exposure to light)
NADH Buffer: 1.5 ml, store at 4°C
20 mM Pyruvate: 0.2 ml, store at -70°C
PEG Solution: 5 ml store at 4°C (viscous pipette with a cut tip)

MSDS:

Tris, PEG

Related Kits:

LDH Assay, PDH Assay, L-Lactate Assay, ATP Assay, NAD+ Assay, alpha-KG Assay, Glutamate Assay, Glutamine Assay