Product Description: Price

Matrix metalloproteinases (MMPs) are secreted or transmembrane endopeptidases belonging to the larger protease family known as the metzincin superfamily. Although MMPs were first found in vertebrates in 1962, they have since been found in invertebrates and plants. All MMPs are synthesized as zymogens requiring extracellular activation, which can be activated in vitro by organomercurials, denaturing agents, and proteases. MMPs control extracellular matrix (ECM) remodeling through their matrix-degrading activities, which play critical roles in many normal growth and developmental processes. Dysregulation of MMP activity has thus been implicated in many pathological conditions. MMP-2 (Gelatinase A, 72 kDa) and MMP-9 (Gelatinase B, 92 kDa) are thought to be particularly important in cancer metastasis. The MMP-2/MMP-9 Assay kit is based on MMP-2 and MMP-9 mediated hydrolysis of Blue Gelatin, which allows for a convenient analysis of MMP activity. In this assay format, MMP activity is dictated by the balance between MMPs and their endogenous inhibitors (TIMPs). MMP activity is determined by optical measurement of 595nm after acetone precipitation of undigested blue gelatin.

#MMP2 #MMP9 #ECM #protease #collagen #gelatin #ECM #matrix

Kit Components:

5x MMP Buffer: 1 ml store at 4ºC

Blue Gelatin: 1 ml (100 reactions), store at -80ºC

SDS:

Tris