Product Description:

Sorbitol dehydrogenase (SDH), found in organisms from bacteria to humans, converts sorbitol, the sugar alcohol form of glucose, to fructose, and the zinc-dependent reaction uses NAD+ as a cofactor. The enzyme along with aldose reductase constitutes the polyol pathway, which connects glucose and fructose metabolism. Organs abundant in SDH activity include the liver, kidney, lens and seminal vesicle. Since SDH activity promotes the formation of NADH, the redox change induced by elevated SDH may have a pathogenic role in certain disease state, making SDH a potential therapeutic target. In addition, SDH has a close evolutionary relationship with alcohol dehydrogenase (ADH). Our non-radioactive colorimetric SDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased SDH activity. The assay can be used for detection of SDH activity in tissue/cell extracts and serum/plasma. The kit is stable for at least one year under proper storage and handling conditions.

Kit Components:

SDH Assay Solution: 5 ml, store at -80ºC (100 wells)

25x SDH Substrate: 0.25 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, acetic acid, Tris

Related Kits:

Glucose Assay, Aldose Reductase Assay