Product Description:
Cellular senescence plays a critical in tumor suppression. Normal mitotic somatic cells gradually cease division after continuous expansion in culture and enter a state referred to as replicative aging or senescence, exhibiting a Hayflick limit of ~50 population doublings (Exp Cell Res 37:614, 1965). It has been suggested that various changes associated with cellular aging may be closely mediated by actin cytoskeleton organization and focal adhesions among cells and extracellular matrix. Senescent cells typically exhibit an enlarged flattened “frying egg” morphology associated with increased activity of acidic beta-galactosidase (J Cell Sci 113:3613, 2000). The increased activity of lysosomal acid beta-galactosidase is usually detected at pH 6, and constitutes the basis of the Senescent Cell Detection assay using the histochemical substrate 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ cell staining. The assay kit is sufficient for 300 - 500 assays. Note that the fixative is not included in the kit.
#Senescent Cell Detection
Kit Components:
10x SCD Buffer: 15 ml, store at 4ºC
Ferricyanide: 10 ml, store at 4ºC
Ferrocyanide: 10 ml, store at 4ºC
Xgal: 4 x 1 ml, store at -20ºC
MSDS:
potasium ferricyanide, potasium ferrocyanide, Xgal, formamide, paraformaldehyde
Related Kits:
beta-Galactosidase (LacZ) Assay, ALP Assay, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
