Product Description: Price

Sorbitol dehydrogenase (SORD), found in organisms from bacteria to humans, converts sorbitol, the sugar alcohol form of glucose, to fructose, and the zinc-dependent reaction uses NAD+ as a cofactor. The enzyme along with aldose reductase constitutes the polyol pathway, which connects glucose and fructose metabolism. Organs abundant in SORD activity include the liver, kidney, lens and seminal vesicle. Since SORD activity promotes the formation of NADH, the redox change induced by elevated SORD may have a pathogenic role in certain disease state, making SORD a potential therapeutic target. In addition, SORD has a close evolutionary relationship with alcohol dehydrogenase (ADH). Our non-radioactive colorimetric SORD assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased SORD activity. The assay can be used for detection of SORD activity in tissue/cell extracts and serum/plasma. The kit is stable for many years under proper storage and handling conditions.

#SorbitolDehydrogenase #polyol #AldoseReductase​

Kit Components:

SORD Assay Solution: 5 ml, store at -80ºC (100 wells)

20x SORD Substrate: 0.25 ml, store at -80ºC

10x Sample Buffer: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Tris

Related Kits:

Glucose Assay, Aldose Reductase Assay