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Plasmid DNA is purified from DH5a strain using our Maxi Plasmid Purification Kit.
Service fee for each plasmid is $160 (20 ug DNA).
Promoter-less Reporter Vectors: Your favorite gene promoter can be inserted into these reporter vectors
p-lacZ beta-galactosidase reporter
p-luc firefly luciferase reporter
p-cat chloramphenicol acetyl transferase
p-EGFP enhanced green fluorescence protein
Promoter-Reporter Vectors: Expression is driven by constitutive eukaryotic promoters/enhancers
pActin-lacZ beta-galactosidase reporter
pCMV-lacZ beta-galactosidase reporter
pRSV-lacZ beta-galactosidase reporter
pSV-lacZ beta-galactosidase reporter
pCMV-luc firefly luciferase reporter
pSV-luc firefly luciferase reporter
pTK-luc firefly luciferase reporter
pActin-cat CAT reporter
pSV-cat CAT reporter
pTK-cat CAT reporter
pCMV-EGFP green fluorescence protein reporter
Eukaryotic Expression Vectors: Your favorite gene can be inserted into the vectors for overexpression
pSV Simian Virus 40 promoter
pMSV Moloney sarcoma virus promoter
pCMV Cytomegalovirus promoter
pADH Alcohol dehydrogenase promoter
pSFFV-neo neomycin selection marker
pCMV-HA for HA tag fusion at the N-terminus
GAL4 Fusion Vectors: These vectors encode a truncated yeast GAL4 DNA-binding domain (1-147), which can be fused
in-frame with your favorite gene. Three different fusion sequences were available (DB1, DB2, DB3) for expression in
higher eukaryotes
pGAL4-DB1 GAL4 fusion protein
pGAL4-DB2 GAL4 fusion protein
pGAL4-DB3 GAL4 fusion protein
Minimum Promoter Vectors: These reporter vectors contain either cat or luc reporters driven by a minimal TATA box or
a minimal TATA box linked to an upstream activating sequence (typically multiple copies of a transcription factor
binding site)
pTATA-luc luciferase reporter driven by a minimal TATA box
pGAL4-TATA-luc luciferase reporter driven by a minimal TATA box and multiple GAL4 binding sites
pGAL4-TATA-cat cat reporter driven by a minimal TATA box and multiple GAL4 binding sites
pSRF-TATA-luc luciferase reporter driven by a minimal TATA box and an SRF binding site
pHSF-TATA-luc luciferase reporter driven by a minimal TATA box and multiple HSF binding sites
pNFkb-TATA-luc luciferase reporter driven by a minimal TATA box and multiple NFkb binding sites
pE12-TATA-luc luciferase reporter driven by a minimal TATA box and multiple E12 binding sites
Yeast Expression Vectors:
pYBD expression of a fusion protein containing the GAL4 DNA-binding domain
pYAD expression of a fusion protein containing the GAL4 activation domain
pRS313 yeast centromere vector with his3 marker
pRS314 yeast centromere vector with trp1 marker
pRS315 yeast centromere vector with leu2 marker
pRS316 yeast centromere vector with ura3 marker
Bacterial Expression vectors:
pGEX Glutathione S-transferase (GST) fusion at the N-terminus
pET3a for protein overexpression
pET3b for protein overexpression
pET3c for protein overexpression
pET3d for protein overexpression
pET14b His tag fusion at the N-terminus
pEGFP expression of EGFP in bacteria
pSP64 bacterial expression
Eukaryotic EGFP Vectors:
pCMV-EGFP-neo expression of EGFP and neomycin selection marker
pEGFP-C1 expression of C-terminal EGFP fusion in higher eukaryotes
pCMS-EGFP coexpression with EGFP in higher eukaryotes
pHyg-EGFP expression of hygromycin-EGFP fusion protein in higher eukaryotes
General Cloning vectors:
pUC8
pUC19
pBR322
pGEM-3z
pGEM-7z
pBluescript
pTZ-19R
Plasmid DNA is purified from DH5a strain using our Maxi Plasmid Purification Kit.
Service fee for each plasmid is $160 (20 ug DNA).
Promoter-less Reporter Vectors: Your favorite gene promoter can be inserted into these reporter vectors
p-lacZ beta-galactosidase reporter
p-luc firefly luciferase reporter
p-cat chloramphenicol acetyl transferase
p-EGFP enhanced green fluorescence protein
Promoter-Reporter Vectors: Expression is driven by constitutive eukaryotic promoters/enhancers
pActin-lacZ beta-galactosidase reporter
pCMV-lacZ beta-galactosidase reporter
pRSV-lacZ beta-galactosidase reporter
pSV-lacZ beta-galactosidase reporter
pCMV-luc firefly luciferase reporter
pSV-luc firefly luciferase reporter
pTK-luc firefly luciferase reporter
pActin-cat CAT reporter
pSV-cat CAT reporter
pTK-cat CAT reporter
pCMV-EGFP green fluorescence protein reporter
Eukaryotic Expression Vectors: Your favorite gene can be inserted into the vectors for overexpression
pSV Simian Virus 40 promoter
pMSV Moloney sarcoma virus promoter
pCMV Cytomegalovirus promoter
pADH Alcohol dehydrogenase promoter
pSFFV-neo neomycin selection marker
pCMV-HA for HA tag fusion at the N-terminus
GAL4 Fusion Vectors: These vectors encode a truncated yeast GAL4 DNA-binding domain (1-147), which can be fused
in-frame with your favorite gene. Three different fusion sequences were available (DB1, DB2, DB3) for expression in
higher eukaryotes
pGAL4-DB1 GAL4 fusion protein
pGAL4-DB2 GAL4 fusion protein
pGAL4-DB3 GAL4 fusion protein
Minimum Promoter Vectors: These reporter vectors contain either cat or luc reporters driven by a minimal TATA box or
a minimal TATA box linked to an upstream activating sequence (typically multiple copies of a transcription factor
binding site)
pTATA-luc luciferase reporter driven by a minimal TATA box
pGAL4-TATA-luc luciferase reporter driven by a minimal TATA box and multiple GAL4 binding sites
pGAL4-TATA-cat cat reporter driven by a minimal TATA box and multiple GAL4 binding sites
pSRF-TATA-luc luciferase reporter driven by a minimal TATA box and an SRF binding site
pHSF-TATA-luc luciferase reporter driven by a minimal TATA box and multiple HSF binding sites
pNFkb-TATA-luc luciferase reporter driven by a minimal TATA box and multiple NFkb binding sites
pE12-TATA-luc luciferase reporter driven by a minimal TATA box and multiple E12 binding sites
Yeast Expression Vectors:
pYBD expression of a fusion protein containing the GAL4 DNA-binding domain
pYAD expression of a fusion protein containing the GAL4 activation domain
pRS313 yeast centromere vector with his3 marker
pRS314 yeast centromere vector with trp1 marker
pRS315 yeast centromere vector with leu2 marker
pRS316 yeast centromere vector with ura3 marker
Bacterial Expression vectors:
pGEX Glutathione S-transferase (GST) fusion at the N-terminus
pET3a for protein overexpression
pET3b for protein overexpression
pET3c for protein overexpression
pET3d for protein overexpression
pET14b His tag fusion at the N-terminus
pEGFP expression of EGFP in bacteria
pSP64 bacterial expression
Eukaryotic EGFP Vectors:
pCMV-EGFP-neo expression of EGFP and neomycin selection marker
pEGFP-C1 expression of C-terminal EGFP fusion in higher eukaryotes
pCMS-EGFP coexpression with EGFP in higher eukaryotes
pHyg-EGFP expression of hygromycin-EGFP fusion protein in higher eukaryotes
General Cloning vectors:
pUC8
pUC19
pBR322
pGEM-3z
pGEM-7z
pBluescript
pTZ-19R
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Biomedical Research Service
& Clinical Application
& Clinical Application