Product Description: Price
Many metabolic reactions are controlled by the energy status of the cell. One index of the energy status is the energy charge (EC) of the cell, which is determined by the concentrations of adenine nucleotides. Although EC could theoretically range in values from 0 to 1, most cells maintain a steady-state EC in the range of 0.8 to 0.95, meaning that the energy status of the cell is buffered. The adenylate nucleotides ATP, ADP and AMP are also allosteric effectors for many enzymes. Intracellular adenine nucleotide concentrations vary during cell growth and cell death. Extracellular adenine nucleotides can regulate various signaling pathways. The assay kit is based on the luciferase reaction in the presence of ATP, which generates yellow-green light that can be conveniently measured by a luminometer or scintillation counter (Anal. Biochem. 80:496,1977). This provides the basis for a sensitive ATP assay system capable of detecting 1 picomole of ATP. The ATP/ADP/AMP Assay Kit is sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. Sample ADP and AMP are converted to ATP by pyruvate kinase- and adenylate kinase-mediated reactions. Note that calculated sample AMP/ADP concentrations may be over- or underestimated due to the multiple factors that can affect nucleotide conversion in vitro. The ATP Assay Solution if stored in aliquots at -80°C is stable for many years. Repeated freeze-thaw cycles should be avoided.
#ATP #ADP #AMP #energycharge #adeninenucleotides
Kit Components:
ATP Assay Solution: 2 x 10 ml, store at -80°C (for 200 assays)
1 mM ATP Standard: 0.1 ml, store at -80°C
ADP-CB: 0.5 ml, store at -80°C
AMP-CB: 0.5 ml, store at -80°C
EDB (Enzyme Dilution Buffer): 5 ml, store at -80°C
ADP-CE: 40 ul, store at 4°C (do not freeze)
AMP-CE: 20 ul, store at 4°C (do not freeze)
4 mM EDTA: 10 ml, store at 4°C
MSDS:
luciferin, EDTA, Tris, DTT
Related Kits:
Cell Proliferation Assay
Citation:
Wong et al
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PLOS ONE 10(8): e0135637. doi:10.1371/journal.pone.0135637, 2015
Yang et al
A pharmacological inhibitor of NLRP3 inflammasome prevents non-alcoholic fatty liver disease in a mouse model induced by high fat diet.
Scientific Reports 6:24399, 2016
Che et al
Loss of BRUCE reduces cellular energy level and induces autophagy by driving activation of the AMPK-ULK1 autophagic initiating axis.
PLoS ONE 14(5): e0216553, 2019
Saber et al
Targeting colorectal cancer cell metabolism through development of cisplatin and metformin nano-cubosomes.
BMC Cancer 18:822, 2018