Product Description: Price
Matrix metalloproteinases (MMPs) are secreted or transmembrane endopeptidases belonging to the larger protease family known as the metzincin superfamily. Although MMPs were first found in vertebrates in 1962, they have since been found in invertebrates and plants. All MMPs are synthesized as zymogens requiring extracellular activation, which can be activated in vitro by organomercurials, denaturing agents, and proteases. MMPs control extracellular matrix (ECM) remodeling through their matrix-degrading activities, which play critical roles in many normal growth and developmental processes. Dysregulation of MMP activity has thus been implicated in many pathological conditions. MMP2 (Gelatinase A, ~70 kDa for latent form) and MMP9 (Gelatinase B, ~90 kDa for latent form) are thought to be particularly important in cancer metastasis. The MMP2/MMP9 assay kit is based on a zymography method for visualizing the gelatin hydrolytic activities of MMP2 and MMP9 present in tissue extracts, cell cultures, serum or urine. After electrophoresis the gel is incubated overnight for MMP activation, during which time gelatin in the region of an active MMP band is digested to fragments sufficiently small to diffuse out of the gel. The gel gives rise to a clear band in the dark blue background upon staining by Coomassie brilliant blue. Please note that the kit does NOT include methanol, acetic acid, and gel apparatus. Proper gel casting technique is required for performing the assay. See gel casting tips at https://www.youtube.com/watch?v=-H8GU_90Gak
#MMP2 #MMP9 #collagen #gelatin #ECM #protease
Kit Components:
Running Gel Solution- 18 ml (5 gels 10x10 cm)
Stacking Gel Solution- 12 ml
Acrylamide Solution- 9 ml (caution: toxic)
AP Solution- 1 ml dH2O (after reconstitution)
TEMED- 0.1 ml (caution: foul smell)
10x Running Buffer- 200 ml (dilute 10x with dH2O)
5x MMP Loading Buffer- 2.5 ml
5x MMP Reaction Solution- 100 ml
50x Calcium: 10 ml
1000x Zinc: 0.5 ml
TX-100: 10 ml
Gelatin: 0.1 g
Gel Staining Solution- 100 ml
SDS:
TX-100, SDS, Tris, acrylamide
Related Kits:
Cathepsin B Assay Kit, Cathepsin C Assay Kit
Citation:
Mastri et al
Secreted Frizzled-related protein 2 as a target in antifibrotic therapeutic intervention
Am J Physiol Cell Physiol, 306:C531, 2014
