Product Description: Price

Matrix metalloproteinases (MMPs) are secreted or transmembrane endopeptidases belonging to the larger protease family known as the metzincin superfamily. Although MMPs were first found in vertebrates in 1962, they have since been found in invertebrates and plants. All MMPs are synthesized as zymogens requiring extracellular activation, which can be activated in vitro by organomercurials, denaturing agents, and proteases. MMPs control extracellular matrix (ECM) remodeling through their matrix-degrading activities, which play critical roles in many normal growth and developmental processes. Dysregulation of MMP activity has thus been implicated in many pathological conditions. MMP2 (Gelatinase A, ~70 kDa for latent form) and MMP9 (Gelatinase B, ~90 kDa for latent form) are thought to be particularly important in cancer metastasis. The MMP2/MMP9 assay kit is based on a zymography method for visualizing the gelatin hydrolytic activities of MMP2 and MMP9 present in tissue extracts, cell cultures, serum or urine. After electrophoresis the gel is incubated overnight for MMP activation, during which time gelatin in the region of an active MMP band is digested to fragments sufficiently small to diffuse out of the gel. The gel gives rise to a clear band in the dark blue background upon staining in Coomassie brilliant blue. We also offer a complementary MMP2/MMP9 Solution Assay (SA) Kit, which can be used for rapid screening of compounds capable of inhibiting MMP. Please note that the kit does NOT include the glass plates, spacers and reagents for gel casting. Customers also need to prepare a Coomassie Brilliant Blue solution for gel staining.

#MMP2 #MMP9 #collagen #gelatin #ECM #protease

Kit Components:

5x MMP Reaction Buffer: 180 ml store at room temperature
5x SDS-PAGE Loading Buffer: 5 ml store at room temperature
50x CaCl2 Solution: 20 ml store at room temperature
1000x ZnSO4 Solution: 1 ml store at room temperature
TX-100: 15 ml store at room temperature
Gelatin: 0.15 g store at room temperature

SDS:

TX-100, SDS, Tris, acrylamide

Related Kits:

MMP2/MMP9 Solution Assay (SA) Kit

Citation:

Mastri et al
Secreted Frizzled-related protein 2 as a target in antifibrotic therapeutic intervention
Am J Physiol Cell Physiol, 306:C531, 2014