Product Description: Price

Hexokinase catalyzes the first step of glycolysis, converting glucose to glucose-6-phosphate. There are four mammalian hexokinase isoforms (I, II, III, and VI) that differ in tissue distribution and catalytic kinetics. Hexokinase I, II, and III exhibit a low Km for glucose whereas hexokinase IV (glucokinase), which is present in the liver and pancreas, has a Km for glucose 100 times higher than that of other hexokinase. The hexokinase assay solution contains 20 mM glucose, and thus measures the total hexokinase activity of crude cell or tissue extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased hexokinase activity. The assay solution should be kept at -80ºC for long-term storage, and will remain stable for many years.

#Hexokinase #Glucokinase #glycolysis #glucose #glucose-6-phosphate​

Kit Components:

Hexokinase Assay Solution: 5 ml (for 100 wells), store at -80ºC

HK Substrate Solution: 0.25 ml, store at 4ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

SDS:

TX-100, DMSO, INT, Tris

Related Kits:

Glucose Assay, GAPDH Assay, Fructose Assay

Citation:

Gershon et al

Hexokinase-2-mediated aerobic glycolysis is integral to cerebellar neurogenesis and pathogenesis of medulloblastoma

Cancer & Metabolism 1:2, 2013

Martin et al

Androgen deprivation leads to increased carbohydrate metabolism and hexokinase 2-mediated survival in Pten/Tp53-deficient prostate cancer

Oncogene 36:525, 2017