Product Description:
Hexokinase catalyzes the first step of glycolysis, converting glucose to glucose-6-phosphate. There are four mammalian hexokinase isoforms (I, II, III, and VI) that differ in tissue distribution and catalytic kinetics. Hexokinase I, II, and III exhibit a low Km for glucose whereas hexokinase IV (glucokinase) has a Km for glucose 100 times higher than that of other hexokinase. The hexokinase assay solution contains 20 mM glucose, and thus measures the total hexokinase activity of crude cell or tissue extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased hexokinase activity. The enzyme assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable indefinitely at -80ºC.
#Hexokinase/Glucokinase
Kit Components:
Hexokinase Assay Solution: 5 ml, store at -70ºC
HK Substrate Solution: 0.25 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
SDS:
TX-100, DMSO, INT, Hepes
Related Kits:
Glucose Assay, GAPDH Enzyme Assay, LDH Enzyme Assay,
L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay
Citation:
Gershon et al
Hexokinase-2-mediated aerobic glycolysis is integral to cerebellar neurogenesis and pathogenesis of medulloblastoma
Cancer & Metabolism 1:2, 2013
Martin et al
Androgen deprivation leads to increased carbohydrate metabolism and hexokinase 2-mediated survival in Pten/Tp53-deficient prostate cancer
Oncogene 36:525, 2017
