Product Description: Price

Luciferase (LUC) encoded by the firefly luciferase gene is widely used as a sensitive reporter enzyme for the study of transcriptional regulation. The enzyme catalyzes, in the presence of ATP (see ATP Assay Kit), the oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by a luminometer or scintillation counter. Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the light flash require consistent timing of the light measurement to obtain reliable data. This provides the basis for an assay system many times more sensitive than the b-galactosidase or other reporter gene systems (Mol. Cell. Biol. 7:725,1987). The luciferase-based reporter assay is thus well suited for those cell systems exhibiting low DNA transfection efficiency. The kit is sufficient for 200 assays using 0.1 ml of Luciferase Substrate per assay. Luciferase Substrate solution if stored in aliquots at -80°C is stable for many years. Repeated freeze-thaw cycles should be avoided.

#Luciferase​ #firefly #DNATransfection #reporter #luciferin #enzyme #promoter

Kit Components:

Luciferase Assay Solution: 2 x 10 ml, store at -80°C

10x Cell Lysis Solution: 25 ml, store at 4°C

SDS:

TX-100, Tris, luciferin, EDTA, DTT

Related Kits:

LacZ Assay

Citation:

Lee et al

Influence of promoter potency on the transcriptional effects of YY1, SRF, and Msc-1 in transient transfection analysis

Nucleic Acids Res 26:3215-3220,1998