Product Description: Price

Glutamate dehydrogenase (GDH or GLDH), an important mitochondrial matrix enzyme integrating carbon and nitrogen metabolism, catalyzes the reversible oxidative deamination of L-glutamate into free ammonia and alpha-ketoglutarate in all tissues. Since the forward reaction generates a TCA cycle intermediate, GDH is involved in energy homeostasis. In addition, kidney GDH participates in acid/base balance of blood by release of ammonia. In clinical diagnostics, increased circulating levels of GDH signal toxic or hypoxic liver damage. Humans have two main GDH isoforms, GDH1 and GDH2, which perform the same core reaction but differ in tissue distribution and allosteric regulation. GDH1 is widely expressed across many tissues, while GDH2 is found in specific cells like brain astrocytes, kidney epithelial cells, and testis Sertoli cells. Our non-radioactive colorimetric GDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GDH activity. The assay is simple and non-radioactive. Each kit is sufficient for 100 wells using a 96-well format. Kit components will remain stable for many years if stored at -80ºC.

#GlutamateDehydrogenase #GDH #GLDH #glutamate #deamination​

Kit Components:

GDH Assay Solution: 5 ml, store at -80ºC (100 wells)

10x GDH Substrate: 0.5 ml, store at -80ºC

10x Cell Lysis Solution: 25 ml, store at room temperature (RT)

SDS:

TX-100, DMSO, INT, Tris

Related Kits:

L-Glutamate Assay, Glutamine Assay

Citation:

Park et al

Identification of multiple Cryptococcal fungicidal drug targets by combined gene dosing and drug affinity responsive target stability screening

mBIO 7:e01073, 2016