Product Description: Price
Mitochondrial malate dehydrogenase (MDH2) catalyzes the last reaction of the TCA Cycle (Krebs Cycle), converting malate and NAD+ to oxaloacetate and NADH in the mitochondria. In reversing the reaction, MDH is also involved in gluconeogenesis, allowing oxaloacetate/malate to leave the mitochondria. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic MDH or MDH1, which is followed by conversion of oxaloacetate to phosphoenolpyruvate by phosphoenolpyruvate carboxykinase (PEPCK). The interconversion between malate and oxaloacetate also constitutes the essential steps of the malate-aspartate shuttle. Mutations in the Krebs cycle enzyme genes are a hallmark of cancer, and MDH2 has been identified as a novel pheochromocytoma and paraganglioma susceptibility gene. In addition, MDH2 mutation causes early-onset severe encephalopathy. MDH exists in most organisms as a homodimeric molecule with subunits weighing between 30 and 35 kDa. The MDH enzyme activity assay kit can be used to quickly monitor potential changes in the activity of MDH during various cellular processes and mitochondrial biogenesis. The non-radioactive colorimetric MDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased MDH activity. The kit is stable for at least one year if handled and stored properly.
#MalateDehydrogenase #MDH #KrebsCycle #TCACycle #gluconeogenesis
Kit Components:
MDH Assay Solution: 5 ml, store at -80°C (100 wells)
10x MDH Substrate: 0.5 ml, store at -80°C
10x Cell Lysis Solution: 25 ml, store at RT
MSDS:
TX-100, DMSO, INT, Tris
Related Kits:
GAPDH Assay, alpha-Ketoglutarate Dehydrogenase Assay, PDC Assay
Citation:
Park et al
Identification of multiple Cryptococcal fungicidal drug targets by combined gene dosing and drug affinity responsive target stability screening
mBIO 7:e01073, 2016
