Product Description:
Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4 subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are therefore possible, depending on the subunit composition (see gel on right). LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased LDH activity. The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product Lactate Assay Kit. The method is adaptable to automation.
#Lactate Dehydrogenase
Kit Components:
LDH Assay Solution: 10 ml, store at -80°C (for 200 wells)
10x Cell Lysis Solution: 25 ml, store at 4ºC
SDS:
TX-100, DMSO, INT, Hepes
Related Kits:
HK Enzyme Assay, GAPDH Enzyme Assay, Glucose Assay,
D-Lactate Assay, PDH Enzyme Assay, G6PD Enzyme Assay Pyruvate Assay, NAD+ assay, L-Lactate Assay
Citation:
Lynch et al
Cell survival under stress is enhanced by a mitochondrial ATP-binding cassette transporter that regulates hemoproteins
Cancer Research 69:5560-5567, 2009
