BMR Lipase Assay Kit

The lipase family of enzymes hydrolyze ester bonds of water-insoluble substrates such as triglycerides, cholesteryl esters, and phospholipids. Mammalian enzymes include pancreatic lipase, hepatic lipase, lipoprotein lipase, and PNPLA members. Pancreatic lipase plays a critical role in lipid digestion. Hepatic lipase hydrolyzes triglycerides and phospholipids present in circulating lipoproteins. Lipoprotein lipase catalyzes the hydrolysis of the triacylglycerol component of circulating VLDL and chylomicrons, which releases free fatty acids for tissue utilization. The PNPLA gene family contains 9 members, among which mutations of PNPLA3 have been strongly linked to various liver diseases such as non-alcoholic fatty liver disease (NAFLD), cirrhosis, and hepatocellular carcinoma (HCC) due to accumulation of triacylglycerol in the liver, i.e., steatosis. In particular, the PNPLA3 I148M mutant exhibits reduced hydrolysis of triacylglycerol and protein turnover, which may contribute to excessive hepatic lipid deposition. Measurement of lipase activity is useful for screening for various GI and inflammatory diseases. The Lipase Assay Kit measures lipase activity using 4-nitrophenyl palmitate as substrate, which upon enzymatic hydrolysis yields the yellow product 4-nitrophenol, which can be conveniently measured by absorbance at 400-410 nm.

#lipase #NASH #NAFLD #steatosis #triglyceride #fattyliver

Kit Components:

Lipase Reaction: 0.1 ml, store at RT (100 wells, shield from light)

Lipase Control: 0.1 ml, store at RT

Lipase Assay Buffer: 10 ml, store at RT

10x Cell Lysis Solution: 25 ml, store at RT

SDS:

Tris, TX-100, nitrophenol palmitate, isopropanol, EDTA

Biomedical Research Service

Lipase Assay Kit