Product Description: Price
Succinate dehydrogenase (SDH) is a four-subunit protein complex (SDHA-SDHB-SDHC-SDHD) embedded in the inner mitochondrial membrane and has a dual role in both the Krebs cycle and the electron transport chain (ETC), thus linking oxidative phosphorylation with the Krebs cycle. It oxidizes succinate to fumarate in the Krebs cycle and transfers electrons from the oxidation of succinate to ubiquinone in Complex II of the ETC. SDH uses electrons from a covalently attached flavin adenine dinucleotide (FAD) cofactor and iron-sulfur clusters to reduce ubiquinone. In addition to the respiratory role, Complex II participates in signaling pathways that control metabolism and cell fate likely through the regulation of cellular levels of succinate. Since SDH is the master controller of succinate levels in eukaryotic cells, changes in Complex II activity can impact succinate-coordinated signaling events such as those mediated by succinate receptor 1 (SUCNR1 or GPR91), which is a promiscuous G protein–coupled receptor. The SDH Assay Kit is based on SDH-mediated oxidation of succinate and reduction of FAD, which is coupled to the reduction of INT to formazan, a water-soluble dye exhibiting an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased SDH activity. Each kit is sufficient for 100 wells using the 96-well format.
#succinate dehydrogenase #Complex II #mitochondrial #respiratory #Krebs cycle #TCA cycle #oxidative phosphorylation #electron transport #fumarate
Kit Components:
20x SDH Substrate: 0.25 ml, store at -80ºC
SDH Assay Solution: 5 ml (100 wells), store at -80ºC
10x Lysis Buffer: 25 ml, store at 4ºC
10x Extraction Buffer: 20 ml, store at 4ºC
SDS:
TW-20, DMSO, INT, Tris
Related Kits:
alpha-KGDC assay, PDC assay, MDH assay, FAO assay, IDH assay
