Product Description: Price

AMP deaminase (AMPD) is a zinc metalloenzyme that catalyzes the deamination of AMP (adenosine monophosphate) to IMP (inosine monophosphate), which plays an important role in purine nucleotide homeostasis, maintenance of energy charge, and muscle function. Three AMPD genes have been identified: AMPD1, AMPD2 and AMPD3, which encode the muscle-, liver-, and erythocyte-specific isoforms, respectively. Alternatively spliced transcript variants encoding the three isoforms are also present. Deficiency of the muscle-specific isoform appears to be a common cause of exercise-induced myopathy and is the most common metabolism-related muscle disorder in the Caucasian population. In addition, certain genetic variations in the AMPD gene may be associated with differences in athletic performance. Interestingly, metformin, a widely prescribed medication for diabetes, inhibits AMPD and this inhibition causes a buildup of AMP, which in turn activates AMP-activated protein kinase (AMPK), thus attenuating hepatic gluconeogenesis, improving insulin sensitivity, and increasing fatty acid oxidation. Additional disease relevance of AMPD comes from the observation that elevated levels of AMPD activity are detected in the brains of Alzheimer's disease patients, which may contribute to the disease's progression due to increased ammonia. The AMPD Assay Kit is based on measurement of ammonia derived from the AMPD-mediated deamination reaction. Kit components are stable for at least one if stored and handled properly.

#AMP deaminase #AMPD #purine catabolism #energy charge #myopathy

Kit Components:

AMPD Reaction- 1 ml (for 100 samples), store at -20ºC

AMPD Control- 1 ml, store at -20ºC

Deaminase Reagent A- 20 ml, store at 4ºC

Deaminase Reagent B- 4 ml, store at 4ºC

10x Cell Lysis Solution- 25 ml, store at room temperature (RT)

MSDS:

TX-100, Salicylic acid, Sodium nitroprusside, Sodium hypochlorite, NaOH

Related Kits:

ADK Assay, NDK Assay, XDH Assay, ADA Assay