Product Description: Price

Adenosine deaminase (ADA) plays an important role in purine metabolism and homeostasis of adenosine/2-deoxyadenosine nucleosides. The enzyme catalyzes the hydrolytic deamination of adenosine, irreversibly deaminating adenosine and converting it to inosine, which can then be deribosylated to hypoxanthine by purine nucleoside phosphorylase. Aside from this metabolic role, ADA participates in several cellular events such as acting as a positive regulator of T-cell activation by interacting with CD26 (DPP4), enhancing dendritic cell immunogenicity, functioning as a positive modulator of the adenosine receptors ADORA1 and ADORA2A, stimulating plasminogen activation, and playing a protective role in post-implantation embryonic development. A lack of ADA leads to a buildup of toxic metabolites that damage developing immune cells, resulting in severe combined immunodeficiency (SCID) and leaving patients vulnerable to recurrent life-threatening infections. Since an elevated level of ADA in bodily fluids often points to an infection or inflammation, ADA test has been used as a diagnostic tool for detection of tuberculosis infections, particularly in pleural effusion. However, high levels of ADA can also be a marker for other inflammatory conditions other than tuberculosis. The ADA Assay Kit is based on measurement of ammonia derived from the deamination reaction. Kit components are stable for at least one if stored and handled properly.

#ADA #adenosine #deaminase #purinemetabolism #SCID #infection #inflammation #tuberculosis

Kit Components:

ADA Reaction- 1 ml (for 100 samples), store at -20ºC

ADA Control- 1 ml, store at -20ºC

Deaminase Reagent A- 20 ml, store at 4ºC

Deaminase Reagent B- 4 ml, store at 4ºC

10x Cell Lysis Solution- 25 ml, store at room temperature (RT)

MSDS:

TX-100, Salicylic acid, Sodium nitroprusside, Sodium hypochlorite, NaOH

Related Kits:

ADK Assay, NDK Assay, XDH Assay, AMPD Assay