Product Description: Price

Tyrosine aminotransferase (TAT) is the first enzyme of a hepatic pathway involved in tyrosine degradation via homogentisate, catalyzing the following reaction: L-tyrosine + alpha-ketoglutarate <-> 4-hydroxyphenylpyruvate + L-glutamate. The TAT gene is regulated by glucocorticoid hormones and cAMP, and alterations in catalytic activity of the enzyme usually parallel changes in enzyme amount. Tyrosinemia (type II or Richner-Hanhart Syndrome, RHS) is the most common metabolic disease caused by TAT mutations (more than 10 identified). RHS patients exhibit elevated blood levels of tyrosine and hydroxyphenylpyruvate accompanied by mental retardation and keratitis (deposition of tyrosine crystals in the cornea). The TAT Assay Kit is based on sequential transamination reaction and glutamate dehydrogenase reaction, which couples the reduction of INT to INT-formazan (molar extinction coefficient = 18/mM-cm at 492 nm), allowing for sensitive detection of TAT enzyme activity. Assay solutions are stable for several years if stored and handled properly.

#TyrosineAminotransferase​ #TAT #Tyrosinemia #Richner-HanhartSyndrome

Kit Components:

TAT Control: 2.5 ml, store at -20°C

TAT Reaction: 2.5 ml, store at -20°C​

TA Assay Solution: 5 ml (for 100 wells), store at -80°C

10x Cell Lysis Solution: 25 ml, store at RT (room temperature)

SDS:

TX-100, DMSO, INT, Tris, NaOH

Related Kits:

AST Assay, ALT Assay, GLDH Assay, ASNS Assay, Glutaminase Assay